DENV NS1 protein, E. coli
Sequence:
MDSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWKQITPELN
HILSENEVKLTIMTGDIKGIMQAGKRSLRPQPTELKYSWKTWGKAKMLSTESHNQTFLIDGPETAECPNTNRAWNS
LEVEDYGFGVFTTNIWLKLKEKQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKIEKASFIEVKNCHWPKS
HTLWSNGVLESEMIIPKNLAGPVSQHNYRPGYHTQITGPWHLGKLEMDFDFCDGTTVVVTEDCGNRGPSLRTTTA
SGKLITEWCCRSCTLPPLRYRGED GCWYGMEIRPLKEKEENLVNSLVTA with polyhistidine tag at the C-terminus
Source:
Escherichia coli
Purity:
>98% as determined by SDS-PAGE. Ni-NTA chromatography
Endotoxin Test:
<0.1 EU/μg by LAL method
Suggested application:
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
SDS-PAGE analysis of recombinant DENV NS1 protein, E. coli
Storage:
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. The protein was lyophilized from a solution containing 1XPBS, pH 8.0. Please use finished within one month after protein reconstitution.
MDSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWKQITPELN
HILSENEVKLTIMTGDIKGIMQAGKRSLRPQPTELKYSWKTWGKAKMLSTESHNQTFLIDGPETAECPNTNRAWNS
LEVEDYGFGVFTTNIWLKLKEKQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKIEKASFIEVKNCHWPKS
HTLWSNGVLESEMIIPKNLAGPVSQHNYRPGYHTQITGPWHLGKLEMDFDFCDGTTVVVTEDCGNRGPSLRTTTA
SGKLITEWCCRSCTLPPLRYRGED GCWYGMEIRPLKEKEENLVNSLVTA with polyhistidine tag at the C-terminus
Source:
Escherichia coli
Purity:
>98% as determined by SDS-PAGE. Ni-NTA chromatography
Endotoxin Test:
<0.1 EU/μg by LAL method
Suggested application:
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
SDS-PAGE analysis of recombinant DENV NS1 protein, E. coli
Storage:
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. The protein was lyophilized from a solution containing 1XPBS, pH 8.0. Please use finished within one month after protein reconstitution.
